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Jackson Laboratory
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ATCC
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ScienCell
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Image Search Results
Journal: Frontiers in Cell and Developmental Biology
Article Title: GFOGER Peptide Modifies the Protein Content of Extracellular Vesicles and Inhibits Vascular Calcification
doi: 10.3389/fcell.2020.589761
Figure Lengend Snippet: GFOGER peptide inhibits Pi-induced calcification: (A,B) in smooth muscle cells. Calcification in smooth muscle cells was induced by incubation with 4 mM Pi in absence or presence of GFOGER peptide or GOERFG peptide. (A) MOVAS cells were incubated with two concentrations of GFOGER peptide (250 and 500 μM) or with 500 μM of GOERFG peptide for 10 days. (B) Primary human vascular smooth muscle cells were incubated with 500 μM of GFOGER peptide for 14 days. Calcification was then measured using the OCP method. (C,D) In rat aortic rings. Rat aortic rings were incubated with 4 mM Pi in presence or absence of 500 μM GFOGER peptide for 10 days. (C) Images of one representative Alizarin Red staining experiment are shown. (D) Intracellular calcium content was quantified by OCP colorimetric method. Data are expressed as mean ± SEM of three independent experiments done in triplicate ( n = 3). *** p < 0.001 vs. 4 mM Pi. Parametric one-way ANOVA test.
Article Snippet:
Techniques: Incubation, Staining